GE RPN1605 User Manual download pdf (Page 19)

Protocol

9. Stop the reaction by the

addition of 5 µl of 0.2 M

EDTA. For use in a

hybridization, denature the

labelled DNA by heating to

95-100°C for 5 minutes, then

chill on ice continued.

Notes

9. Continued

described in Appendix III.

Calculation of probe specific

activity is described in

Appendix II.

Extensive experimentation

with Rapid-hyb buffer

(RPN1635/6) has shown that

probe purification, even

under the conditions given

above is not required with

the isotopes

P and

Purification of

S labelled

probes is however required

to reduce filter background.

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GE RPN1605 User Manual Page 19