GE RPN2510E User Manual download pdf (Page 14)

Appendix 1. Products for

electrophoresis

DNA Markers

Precise Sizing Digest of Natural DNAs

50 Base-pair 100 Base-pair KiloBase DRigest III

ladder ladder DNA marker 27-4060-01

27-4005-01 27-4007-01 27-4004-01

500

2000

10000 23130

450 1900 8000 9416

400 1800 6000 6557

350 1700 5000 4361

300 1600 4000 2322

250 1500 3000 2027

200 1400 2500 1353

150 1300 2000 1078

100 1200 1500 872

50 1100 1000 603

1000 500 564

900 310

800 281

700 271

600 234

500 194

400 125

300 118

200 72

100

a not necessarily the largest size fragment possible, only the largest that is

readily distinguishable

b cos ends are located on these bands

c may not always be visible

Marker code number Recommended gel Loading amount (µg/lane) Heat before loading

27-4005-01 2% agarose/6% polyacrylamide 2.0 No

27-4007-01 1.5% agarose 2.0 No

27-4004-01 0.8% agarose 0.5 No

27-4060-01 1% agarose 0.5–1.0 60–65°C for 2 minutes

RPN2510EUM Appendix 1 Rev C 06/2007 14

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GE RPN2510E User Manual Page 14